Meyer, M. & Kircher, M. Illumina sequencing library preparation for highly multiplexed target capture and sequencing. YOUTH VAPING: AN ANALYSIS OF AN EPIDEMIC 6 Youth Vaping: An Analysis of an Epidemic 'Vape.' In the 1980s, this was a phrase used to describe weapon uselimited primarily to science fiction writing. In the MDS plot, the Le Piage II individual falls particularly close to individuals belonging to the Fournol cluster, suggesting a local genetic continuity of this ancestry during the LGM (Supplementary Fig. Gravettian cranial morphology and human group affinities during the European Upper Palaeolithic. Error bars show 1s.e.m. Gonzlez-Fortes, G. et al. My experience includes an internship at Goldman Sachs where I did some machine learning work before I took up my current data science job in tech. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in To mark the occasion, rather than doing some data analysis or looking at trends, we wanted to dedicate this bulletin to seven women in tech, data, and science that you should know about. In particular, the 13ka Pradis 1 individual from northeastern Italy represents the most basal lineage compared to all other Epigravettian-associated individuals, including the older Tagliente 2 and Villabruna genomes from northern Italy (group 1). 2022 Weird Science Plus - All Rights Reserved. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. During this time agriculture and a sedentary lifestyle spread from Anatolia to Europe. Protoc. 7 and Supplementary Table 3), which coincides roughly with the first appearance of the Oberkassel ancestry in central Europe. Previously, she has interned at Goldman Sachs where she used Python and Scala to implement machine learning techniques for automated anomaly detection. Libraries were quantified using the IS7 and IS8 primers59 in a quantification assay using a DyNAmo SYBR Green qPCR Kit (Thermo Fisher Scientific) on the LightCycler 480 (Roche). Reimer, P. J. et al. Publishers note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Meyer, M. et al. This is first detected in an individual from Gross Fredenwalde in northeastern Germany and reaches around 10% in most European hunter-gatherer individuals thereafter (Extended Data Fig. Sci. The idea of data warehousing is to store large amounts of data in a way that makes it easier to extract and analyse. Much attention has b Fluorescent probes targeting specific organelles are useful tools for understanding their intracellular behaviors. The x-axes report the geographic regions as divided in Fig. YSYS stands for Your Startup, Your Story and is dedicated to connecting diverse people with opportunities in tech.. Happy International Womens Day! Limits of long-term selection against Neandertal introgression. 116, 105100 (2020). 276, 107319 (2022). Tina Huang Popularity . This study focuses on the people who lived between 35,000 and 5,000 years ago and that are, at least partially, the ancestors of the present-day population of Western Eurasia, including -- for the first time -- the genomes of people who lived during the Last Glacial Maximum (LGM), the coldest phase of the last Ice Age, around 25,000 years ago. PLoS ONE 14, e0217996 (2019). Modern humans began to spread across Eurasia around 45,000 years ago but previous research showed that the first modern humans that arrived in Europe did not contribute to later populations. Lead Advisor - Data Science. 2010, pdb.prot5448 (2010). Ice Age survivors -- ScienceDaily A series of f4-symmetry statistics confirm that all the Epigravettian-associated individuals are cladal, and do not share excess affinity with any local (Paglicci 12) or non-local preceding ancestries (Goyet Q116-1, Kostenki 14, Malta 1 or Vstonice) (Supplementary Data 2.F). Sci. Genetically, however, the populations from western and southwestern Europe (today's France and Iberia) differed from contemporaneous populations from central and southern Europe (today's Czech Republic and Italy). Djindjian, F. Territories and economies of hunter-gatherer groups during the last glacial maximum in Europe. However, the level of genetic continuity between individuals associated with these cultures and the population structure among Epigravettian-associated individuals have not been fully explored. Its mainly used in industries such as finance, insurance, healthcare, and insurance-related apps such as credit card applications, vehicle purchasing, etc. In many cases, this means loading data into a relational database (such as MySQL, MS SQL Server, or Oracle). The key areas to focus on when getting started in data science [6:01] Tina's five steps to consistently doing anything [11:55] Tina's day-to-day life as a data scientist at one of the world's largest tech companies [20:02] How Tina's computer science background helps her work [26:20] Traditional banking culture vs big tech [32:12] b, Calibrated radiocarbon dates of individuals plotted in a. under exclusive license to Springer Nature Singapore Pte Ltd. 2022 R. Huang et al. Catalano, G. et al. You need more data until you have the right ingredients to make a good recipe, but you also need less data until you have the right amount of ingredients to make a bad recipe. In addition to her data science work, Tina has also created the popular popular game Code Wars. Olalde, I. Adv. In addition to her rich experience, including bioinformatics cancer research at OICR, Tina is a . Science 360, eaar7711 (2018). - Technical researcher and data analyst under the supervision of Dr. A. Meyer and Dr. S. Bae at UCLA. Tina Huang. 4a). If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Consultant - October 2015 to June 2016. The baseline of each population was determined using the average heterozygosity rate of individuals estimated from pseudo-diploid genotypes (Supplementary Data 1.E). CAS Epoxy Flooring UAE; Floor Coating UAE; Self Leveling Floor Coating; Wood Finishes and Coating; Functional Coatings. 9, 442 (2018). HaploGrep 2: mitochondrial haplogroup classification in the era of high-throughput sequencing. Tina started her YouTube channel in 2020. Google Scholar. 12). 2c). Biol. Highly recommended for not only people preparing for interviews but for people that just want to improve their coding skills. A user manual including an installation guide is available at https://haproh.readthedocs.io/en/latest/hapROH_with_contamination.html. 5). However, published Gravettian-associated genomes originate from central and southern Europe, leaving the genetic profile of Gravettian-associated humangroups from western and southwestern Europe undescribed. However, the extent to which groups associated with the Solutrean culture are in genetic continuity with earlier and later populations from the same region is unknown because no genomic data from Solutrean-associated individuals have been reported previously. Junior Data Scientist May 2017 - April 2018. 4, 324333 (2020). The analysis of biological relatedness was performed by calculating relatedness coefficient (r) based on PMR on the autosomal SNPs (Supplementary Data 1.F and Supplementary Information, section4). Nucleic Acids Res. Powered by Symplectic part of Digital Science . Become an in-demand #DataScience professional TODAY with the 365 Data Science Program -> https://365datascience.com, analyzing similar products to compare how they performed, data visualizations with SQL and Python or R. Tina Huang: Data science, computer science, and career advice expert. 581582, 128163 (2021). Commun. Olalde, I. et al. Curr. Proc. The lsqproject: YES parameter was used to minimize the effect of missing data in ancient individuals. Quat. Applied Sciences | Free Full-Text | Comparison of Perioperative Instead, for the two SNPs associated with skin colour (SLC24A5 (rs1426654) and SLC45A2 (rs16891982)), Sidelkino clusterand Ukraine HG groups show a higher frequency (>90% for SLC24A5 and 2961% for SLC45A2) of the derived alleles related to light skin colour, compared with Oberkassel and Villabruna clusters, where those alleles are almost completely absent (<1%). F4-statistics also show that the approximately8.2ka Yuzhniy Oleniy Ostrov group from Karelia in western Russia formed by 19 genomes has comparable or lower affinity to Villabruna ancestry than all the other EHG groups (Supplementary Data 2.K). The emergence of hunter-gatherer pottery in the Urals and West Siberia: new dating and stable isotope evidence. Newsletter, I also have a newsletter called Boop's Keyboard! Dots are maximum likelihood estimates and error bars show 95% confidence intervals of the derived allele frequencies (n, the number of individuals in each group, is provided in Supplementary Data 3.G). The three squares highlight Baltic HG groups associated with the Comb Ceramic Culture (CCC) that show a marked increase in Sidelkino-related ancestrycompared to olderBaltic HG groups. As of right now, it doesnt seem that MySQL is the right choice for storing data because the database is so large, it would be impossible to find and query the necessary information. She has been working for the data sciences industry since 2014. Res. Tina Huang I'm a data scientist, youtuber, and the first Lonely Octopus . We used the default posterior threshold of 0.01, and mapping and sequencing quality cutoffs of 30. 5 and Extended Data Fig. Teaching Assistant. However, using f4-statistics, we show that all Magdalenian-associated individuals, and not only El Mirn, carry Villabruna-related ancestry whencompared tothe Fournol cluster(Supplementary Data 2.H). 2022-112-4f84b - Ecologizr - Chapter 2 Science Education in Algeria PubMed Science 366, 708714 (2019). Okenla is also part of Atomicos Angel Programme 2021, and is an advisory board member for both Coders of Colour and the No.10 Innovation Fellowship. Last Name Huang #8. A recent bottleneck of Y chromosome diversity coincides with a global change in culture. Mathieson, I. et al. Illumina sequencing platforms were also used to sequence the 1240k and mtDNA captured libraries (Supplementary Data 1.B). ScienceDaily, 1 March 2023. Paleogenomic evidence for multi-generational mixing between Neolithic farmers and Mesolithic hunter-gatherers in the Lower Danube Basin. The horizontal dashed line marks the boundary between Late Pleistocene and Holocene. No need to do it alone our community of learners and instructors is there for you. Prfer, K. et al. By using our site you agree toour Ecol. They were linked by a common archaeological culture: they used similar weapons and produced similar portable art. Kircher, M., Sawyer, S. & Meyer, M. Double indexing overcomes inaccuracies in multiplex sequencing on the Illumina platform. The IntCal20 Northern Hemisphere radiocarbon age calibration curve (055 cal kbp). Resurrected Supernova Provides Missing-Link, Bald Eagles Aren't Fledging as Many Chicks, Ultracool Dwarf Binary Stars Break Records, Deflecting Asteroids to Protect Planet Earth, Quantum Chemistry: Molecules Caught Tunneling, Shark from Jurassic Period Highly Evolved, 2.9-Million-Year-Old Butchery Site Reopens Case of Who Made First Stone Tools, Gut Microbes Can Boost the Motivation to Exercise, Warmer Climate May Drive Fungi to Be More Dangerous to Our Health, Seawater Split to Produce 'Green' Hydrogen. and H.F. produced a subset of the radiocarbon dates. Runtime Collective Limited (trading as Brandwatch). The oldest individual in the Fournol cluster is Ormesson 2988 from northeastern France (31ka, Early/Middle Gravettian), whereasa Gravettian group from Goyet in Belgium (27ka, Late Gravettian) is found to be a mixture between the Vstonice and Fournol clusters. Tina Huang is a statistician who focuses on the statistical modeling and analysis of big data. Genome Biol. 62, 3643 (2020). Cationic Oligopeptides with Amino Groups as Synthetic Nucleolar Curr. Twelve-month follow-up after hospitalization for SARS-COV-2: physiology On the basis of the genetic variation of present-day Europeans, this could imply phenotypic differences between post-14ka hunter-gatherer populations across Europe, with individuals in the Oberkassel cluster possibly exhibiting darker skin and lighter eyes, and individuals in the Sidelkino cluster possibly lighter skin and darker eye colour. Evol. Consistent with previous findings, none of the analysed groups show the derived allele at SNP rs4988235 on the LCT gene, which is responsible for lactase persistence. Srie Prhistoire dEurope 9 (ditions, 2001). The authors thank G. Marciani and O. Jris for comments on archaeology; C. Jeong, M. Spyrou and K. Prfer for comments on genetics; M. OReilly for graphical support for Fig. You probably remember me from class or an event, and that's why this profile is so interesting - it traces my journey from student-athlete at the University of California Davis into a successful entrepreneur with multiple ventures under her belt by age 25, Your email address will not be published. Both double-stranded and single-stranded libraries were further amplified with IS5/IS6 primers to reach a concentration of 200400ng/l as measured on a NanoDrop spectrophotometer (Thermo Fisher Scientific). Hi! 1 and names are indicated next to the symbols. Natl Acad. The increasing level of admixture between distinct hunter-gatherer populations from around 8ka onwards indicates an intensified mobility of those forager groups. This might also explain the genetic uniformity of the Oberkassel cluster across large parts of western Eurasia but genomic data from between 15 and 14ka is needed to understand the exact dynamics of this turnover. It involves lots of hypothesis testing and statistics which 365 Data Science also has a wonderful course on. Yang, M. A. et al. Join now Sign in . @hellotinah. Bioinformatics 20, 289290 (2004). "The data we gained from this study provides us with astonishingly detailed insights into the developments and encounters of West Eurasian hunter-gatherer groups," Posth summarises. Natl Acad. Natural Language Processing (NLP) is another technique that has a well-deserved position in product development. Natl Acad. Third, Magdalenian-associated individuals not only from Iberia but also from the rest of Europe carry Epigravettian-associated ancestry (Villabruna cluster). 2022 Fab Business Solutions - All Rights Reserved. It was awesome seeing the thought process behind each of the questions - it helps students immensely! In her journey of eventually becoming a data scientist, Tina started out with an undergraduate in Pharmacology. Article Quat. At the same time, these two groups started mixing with each other, and continued to do so for around 3,000 years," Krause says. Nature 528, 499503 (2015). 1). In anticipation of possible near-term approval from the US Food and Drug Administration for renal denervation, there must be an insistence on longer-term clinical outcomes and safety data. Bronk Ramsey, C. Bayesian analysis of radiocarbon dates. Check out The data science resume that got me my FAANG job and subscribe to Tina Huangs YouTube channel. A 23,000-year-old southern-Iberian individual links human groups that lived in Western Europe before and after the Last Glacial Maximum. Dr. Huang taught General Chemistry, Quantitative Analysis, and Instrumental Methods for eight years at . Danielle Fishel. I admit, i was too confident in my SQL, however that changed after seeing the interview questions that could be asked from different angle, the most important thing i have learnt in this course is CTE, and her Sunday SQL videos on YouTube is icing on cake. Tina Huang (@hellotinah) / Twitter Evol. R. Soc. Its no surprise that she was selected for the Code Cup, a yearly event where students from all over the world compete in coding challenges. Freshwater radiocarbon reservoir effects at the burial ground of Minino, Northwest Russia. The expansion of farming by9,8, 7.5 and7 kais shown as green shades. Enjoy maximum flexibility and forget about cramming against the clock. Extended Data Fig. This means you will have no set time limit for completing any of the modules or assignments. I have been watching Tina's YouTube channel's videos since the past month and find her explanations / reviews very useful and actionable and being in Toronto myself I think it will be same contextually when I start applying for DSc jobs (and make the big switch from Healthcare that I am in currently). This course will help you build a deliberate strategy when participating in a data scientist job interview involving SQL. H. Rougier and I.C. Living up to Expectations: The Strengths and Challenges Experienced by Then the cleaned reads together with cleaned reads from 1240k capture sequencing were mapped to human reference mitochondrial sequence NC_012920.1 with BWA 0.7.12 aln/samse algorithm (parameters n 0.01, l 16500) and realigned with CircularMapper64. Biol. USA 110, 22232227 (2013). This course is presenting itself as both a refresher and a challenge. For the placement of individuals onto a Y-chromosomal phylogenetic tree (Extended Data Fig. Search 210,843,632 papers from all fields of science. Genetics 192, 10651093 (2012). Then we combined our newly generated genotypes with published genotypes from ancient and modern individuals from AADR v42.4 (Allen Ancient DNA Resource (https://reich.hms.harvard.edu/allen-ancient-dna-resource-aadr-downloadable-genotypes-present-day-and-ancient-dna-data) version 42.4) for downstream analysis1,4,7,14,16,23,36,37,38,39,40,42,43,45,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93,94. Its like the different ingredients in your recipe. Tina Huang Profile page. As previously hinted37, we find a large frequency variation in alleles related to skin and eye pigmentation among post-LGM hunter-gatherer groups. Contact Us. Protoc. Tina Huang - Climate & Environment Knowledge Analyst - LinkedIn "Further interdisciplinary research will clarify which exact processes were responsible for the genetic replacements of entire Ice Age populations.". Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. The data generated in this study enabled us to investigate genomic transformations of and interactions between Eurasian hunter-gatherers at high resolution (Extended Data Fig. Everything you need to know is included in the course. We visualize the total amount of ROH longer than 4 cM for (a) pre-LGM individuals, (b) Epigravettian- and Magdalenian-associated individuals, (c) individuals carrying high proportions of Sidelkino-related ancestry, and (d) individuals carrying high proportions of Oberkassel-related ancestry. How to Become a Data Scientist at FAANG (Ft. Tina Huang) - Morioh Publication: Huang T, Reimand J. Network-rewiring mutation in kinase signaling networks are informative of cancer patient. The Sidelkino ancestry also emerged around 14ka with its first direct evidence in eastern Europe43 dated to 13ka. Libraries showing a mitochondrial or nuclear contamination rate over 10% were considered substantially contaminated whereas those between 5 and 10% were considered marginally contaminated and were treated differently (details are provided in Supplementary Information, section3). Natl Acad. "We find that individuals associated with a later culture, the Epigravettian, are genetically distinct from the area's previous inhabitants," says co-author He Yu. & Strimmer, K. APE: analyses of phylogenetics and evolution in R language. Total students. Amina is a software engineer, who is also an accessibility advocate and content creator. Billed once. Nature 514, 445449 (2014). The ROH segments in hunter-gatherer genomes were identified using hapROH101. In spite of growing discussions about its nature27,28, the Epigravettian culture has been traditionally assumed to be the result of a transition from the preceding local Gravettian29. The resulting GoyetQ2 cluster includes individuals spanning from western France to Poland in the period between 18 and 15ka. Google Scholar. Science 344, 747750 (2014). STOC 2023: Accepted Papers 8). Colour legend is shown in (e). Skoglund, P. et al. As she gains working experience with one of the FAANG companies, Tina continues to share interesting details about her career journey with the audience. Nature 570, 182188 (2019). Max Planck Institute for Evolutionary Anthropology. NEWSLETTER: https://tinahuang.substack.com/ It's about learning, coding, and generally how to get your sh*t together c: I've been a FAANG data scientist f. Join a growing community of 11K+ to find accountability partners, take part in productivity sprints, and join discord only events! Home Courses Scoreboard Recommendations. Evol. Youll be surprised how much value there is in a quick XGBoost model, for example. The f4-statistics were calculated by qpDstat 755 with parameter f4 mode: YES, with the Mbuti.DG population from Africa used as outgroup in all f-statistics analyses. Can you tell I had fun filming this episode with Tina Huang, YouTube data science superstar (293k subscribers)? 3.Tina Huang. Sci. Nat. Bioinformatics 29, 16821684 (2013). Then we merged the genotypes from different libraries of the same individual, by randomly picking alleles from available genotype calls, using a custom script. . Max Planck Institute for Evolutionary Anthropology. PubMed BMC Bioinformatics 15, 356 (2014). National Science Council feb. 2009 - feb. 2009 1 maand. My name is Tina Huang and I'm a data scientist at a FAANG company. After the LGM, a genetic component distantly linked to the Goyet Q116-1 individual from Belgiumdated to 35 kanamed GoyetQ2 ancestry(hereafter, GoyetQ2 cluster or ancestry)reappeared in individuals from southwestern and central Europe associated with the Magdalenian culture (1914ka from Iberia to eastern Europe across central Europe) and in an admixed form in subsequent Final Palaeolithic and Mesolithic hunter-gatherers4,14, but the geographic extension of this ancestry is still unclear. Empleos Personas Learning . In addition, those groups also carry affinity to ANF suggesting more complex genetic processes behind their demographic history16. 4a and Supplementary Data 3.C). Posth, C. et al. The inferred pattern of short ROH (48 cM, visualized in blue) being common is in stark contrast to most later farmer populations, where the majority of individuals have no short ROH whatsoever (see101), and evidences small effective population sizes across West Eurasian hunter-gatherer groups. Utilized python and R on high performance computing Linux cluster to discover 3 new cancer mutations. In this debated framework, Gravettian-associated individuals have been suggested to represent a biologically homogeneous population on the basis of craniometric and genomic data4,22. and JavaScript. USA 110, 1575863 (2013). CAS 9). Jensen, T. Z. T. et al. Fu, Q. et al. Li, H. et al. This is in sharp contrast to the genetic history of Iberian hunter-gatherers, where the spread of the Villabruna/Oberkassel ancestry involved multiple local admixture events with groups carrying high proportions of GoyetQ2 ancestry14 (Fig. Curr. I love learning new things a. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers,who were also characterized by marked differences in phenotypically relevant variants. The previously described Vstonice cluster, including a newly reported 29,000-year-old individual from Paglicci cave (Paglicci 12) in southern Italy, is closely related to the previously published genomes from Sunghir and Kostenki 12 in western Russia, which are dated to 34ka and 32ka, respectively4,23. Amazing content! Data Professor - How to Become a Data Scientist at FAANG - Facebook After 2 washing steps using the manufacturers wash buffer, DNA was eluted in TET (10mM Tris, 1mM EDTA and 0.05% Tween) in two steps for a final volume of 100l. Here, data really gets to shine, in that its both the source of truth and the driver of insights. A dynastic elite in monumental Neolithic society. 272, 288296 (2012). Radiocarbon 55, 163177 (2013). In our case, we had data about people who had fallen off the internet, and we wanted to predict the probability that they would fall off at a certain time. Previous studies identified two different genetic compositions in Magdalenian-associated individualsthe GoyetQ2 cluster including central-western European genomes dated to around 15ka (from France, Belgium and Germany), and the ancestry of the El Mirn individual from Spain4,14 from around 19ka. A phylogeographic reconstruction of different lineages carrying this ancestry further suggests its entry point into northeastern Italy from the Balkans followed by a north-to-south expansion into the Italian peninsula alongside a population decline through sequential bottlenecks.
